Coding
VVD-YN

Part:BBa_K1616002:Design

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-17)

VVD linked to YN155 (YFP Nter split) with double terminator T7



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of VVD had 2 illegal sites PstI; that have been removed. Also, the N terminal of YFP had 1 illegal site PstI


Source

This part have been created thank to gblock, our team have assembled the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the N terminal of YFP split(1). The downstream part of this composite part is double T7 terminator (BBa_B0015) and the upstream is the RBS (BBa_J61100).


References

(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan

Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell. 2002;9(4):789–98.